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Alternative_name
FITC-Asp-Glu-Val-Asp-fluoromethyl ketone; FITC-D(OMe)-E(OMe)-VD(OMe)-FMK
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Description
FITC-D(OMe)E(OMe)VD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable caspase inhibitor D(OMe)E(OMe)VD(OMe)-FMK that acts as an in situ marker for detection of caspase-3 in living cells. FITC-D(OMe)E(OMe)VD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. The bound marker is localized by fluorescence detection
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Structure Available
Yes
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Peptide sequence
FITC-D(OMe)-E(OMe)-VD(OMe)-FMK
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Salt Form
No
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Molecular Formula
C₄₃H₄₆FN₅O₁₅S
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Molecular Weight
923.91
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Handling
Protect from air and moisture
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Tag Line
A caspase-3 in situ marker
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
36 months
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SMILES
O=C(OC)CC[C@H](NC([C@@H](N/C(S)=N/C1=CC=C(C2(C3=CC=C(O)C=C3OC4=C2C=CC(O)=C4)OC5=O)C5=C1)CC(OC)=O)=O)C(N[C@@H](C(C)C)C(N[C@H](C(CF)=O)CC(OC)=O)=O)=O
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InChi
InChI=1S/C43H46FN5O15S/c1-20(2)37(40(58)47-29(31(52)19-44)17-35(54)61-4)49-38(56)28(12-13-34(53)60-3)46-39(57)30(18-36(55)62-5)48-42(65)45-21-6-9-25-24(14-21)41(59)64-43(25)26-10-7-22(50)15-32(26)63-33-16-23(51)8-11-27(33)43/h6-11,14-16,20,28-30,37,50-51H,12-13,17-19H2,1-5H3,(H,46,57)(H,47,58)(H,49,56)(H2,45,48,65)/t28-,29-,30-,37-/m0/s1
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InChi Key
GRXLYHYCYCURGM-OWBZZYPYSA-N
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MSDS Available
Yes
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
