FGF-acidic Recombinant Protein
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Catalog number
40-159
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Price
Please ask
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Size
0.01 mg, 0.05 mg
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About recombinant protein
FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.
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Other name of the recombinant
Fam, Fgfa, Dffrx, Fgf-1, Fibroblast growth factor 1, Acidic fibroblast growth factor, FGF-1
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Recombinant in
E. coli
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SEQUENCE
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
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Activity
Assay 1: The ED50 was determined by the dose - dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/mL, corresponding to a specific activity of ≥ 1 x 105 units/mg.Assay 2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of ≥ 2 x 106 units/mg.
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Keep at
The lyophilized FGF-acidic recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted FGF-acidic is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing FGF-acidic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
6753850
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Accession nr
NP_034327.1
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Pureity
Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
14164
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Gene name
fibroblast growth factor 1
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Source
Mus musculus
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Uniprot
P61148
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Fgf1
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Short name
FGF-acidic Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
FGF-acidic Rec. Protein
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Alternative technique
rec
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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