FasL Antibody (Fas Ligand)

• Catalog number
  R30652
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  FasL (Fas Ligand)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the FasL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This FasL antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P48023
• Purity
  Antigen affinity
• Description
  Fas ligand (FasL, CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the target cell. This trimerization usually leads to apoptosis, or cell death. Soluble protein is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
• Immunogen
  An amino acid sequence from the C-terminus of human Fas Ligand (ELSLVNFEESQTFFGLYKL) was used as the immunogen for this FasL antibody.
• Storage
  After reconstitution, the FasL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
• French translation
  anticorps

• Gene target
  FasL   Fas   Ligand  
• Gene symbol
  FASLG
• Short name
  Anti-FasL (Fas Ligand)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to FasL (Fas Ligand)
• Alternative technique
  antibodies
• Alternative to gene target
  Fas (TNF receptor superfamily, member 6), ALPS1A and APO-1 and APT1 and CD95 and FAS1 and FASTM and TNFRSF6, FAS and IDBG-81669 and ENSG00000026103 and 355, identical protein binding, nuclei, Fas and IDBG-159577 and ENSMUSG00000024778 and 14102, FAS and IDBG-637356 and ENSBTAG00000010785 and 282488

Gene info

• Identity
  HGNC:11936
• Gene
  FASLG
• Long gene name
  Fas ligand
• Synonyms gene
  • APT1LG1
  • TNFSF6
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 6
  • Fas ligand (TNF superfamily, member 6)
• Synonyms
  • FasL
  • CD178
• GenBank acession
  • U11821
• Locus
  1q24.3
• Discovery year
  1994-12-09
• Entrez gene record
  356
• Pubmed identfication
  • 7826947
  • 9022072
• Classification
  • Tumor necrosis factor superfamily
  • Death inducing signaling complex
  • CD molecules
• VEGA ID
  OTTHUMG00000034841
• Locus Specific Databases
  • FASLGbase: Mutation registry for Autoimmune lymphoproliferative syndrome, type 1B (ALPS1B) (previously known as TNFSF6base)
  • LRG_58

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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