EZScreen™ NAD+/NADH Colorimetric Assay Kit (384-well)

  • Catalog number
    K958-400
  • Price
    Please ask
  • Size
    400 assays
  • Description
    Highly Sensitive, minimum sample preparation required
  • Summary
    • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.
  • Detection Method
    Absorbance (450 nm)
  • Species Reactivity
    Species reactivity- Mammalian
  • Applications
    BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio
  • Sample Type
    Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
  • Features Benefits
    • Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
  • Short name
    EZScreen™ NAD+/NADH Colorimetric Assay Kit (384- )
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    EZScreen™ Nicotinamide adenine dinucleotide+/NADH colorimetry test reagent (384-well)
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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