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Synonyms
Protein Extraction
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Alternative_names
Lysozyme, EZLys™ , 1,4-beta-N-acetylmuramidase C
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Description
An enzyme which breaks down bacterial cell wall
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥85%
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Assay
SDS-PAGE
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Unit Definition
>100,000 units/mg protein. A unit (U) is defined as the amount of enzyme which will reduce the absorbance at 450 nm, using a suspension of Micrococcus lysodeikticus as a substrate in a 2.6 ml reaction volume, by 0.001 absorbance units per min at pH 6.24 and 25°C.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Off- white powder
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Physical form description
Off- white powder
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Reconstitution Instructions
Clear solution at 10 mg/ml in PBS, pH 7.4 or H₂O
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Background Information
EZLys™ Lysozyme is a recombinant human lysozyme expressed and purified in an animal-free system (rice). It has an identical amino acid sequence and physic-chemical properties of native lysozyme. The enzyme cleaves b (1,4) linkage between N-acetylglucosamine and N-acetylmuramic acid of the peptidoglycan component in the bacterial cell wall. It exhibits four times more activity than commercial chicken lysozyme and is useful in the recovery of proteins expressed in bacteria (e.g. E. coli). EZ Lysozyme is designed for gentle cell lysis which leads to a minimal risk of protein denaturation.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
