EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant

  • Catalog number
    7868-2500
  • Price
    Please ask
  • Size
    2500 Units
  • Synonyms
    Ulp1 peptidase, SUMO Protease
  • Alternative_names
    Ulp1 peptidase, SUMO Protease
  • Description
    >90% Pure, Active, Yeast Recombinant, His-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
  • Biological activity
    The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
  • Results
    ≥ 1 X 106 units/mg.
  • Unit Definition
    One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.
  • Molecular Weight
    28.7 kDa (403-621 aa + N-terminal Poly-His tag).
  • Storage Temp
    -80°C
  • Shipping
    Dry ice
  • Shelf Life
    12 months
  • Concentration
    0.1 mg/ml (100 U/µl)
  • Appearance
    Liquid
  • Physical form description
    0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
  • Background Information
    SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).
  • Amino acid sequence
    403-621 aa + N-terminal Poly-His tag
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Conjugation
    histidine
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    SUMO1
  • Short name
    EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    Yeast, Ascomycota
  • Label
    his-tagged
  • Species
    Yeasts
  • Alternative name
    EZCut™ SUMO Protease 1 (histidine-tagged), Yeast Rec.
  • Alternative technique
    rec
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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