EPO-alpha, human recombinant

• Catalog number
  4763-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Erythropoietin-α, EPO-α, Epoetin, EP, MGC138142
• Alternative_names
  Erythropoietin-α, EPO-α, Epoetin, EP, MGC138142
• Description
  A glycoprotein hormone that controls erythropoiesis
• Recombinant
  Yes
• Source
  CHO cells
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥90%
• Biological activity
  Determined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.
• Molecular Weight
  37 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Each mg of lyophilized protein contains 0.58 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers containing a carrier protein (like 0.1% BSA) and stored in working aliquots at -20°C to -80ºC for future use.
• Background Information
  Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. The differentiation of CFU-E (Colony Forming Unit -Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. BioVision’s Human EPO contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  The EPO-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  EPO-alpha  
• Gene symbol
  EPO, TIMP1, EPX
• Short name
  EPO-alpha, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  erythropoietin-a, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  erythropoietin, EP and MVCD2, EPO and IDBG-31941 and ENSG00000130427 and 2056, protein kinase activator activity, Extracellular, Epo and IDBG-205003 and ENSMUSG00000029711 and 13856, EPO and IDBG-640493 and ENSBTAG00000003430 and 280784

Gene info

• Identity
  HGNC:3415
• Gene
  EPO
• Long gene name
  erythropoietin
• Synonyms
  • EP
• GenBank acession
  • X02157
• Locus
  7q22.1
• Discovery year
  2001-06-22
• Entrez gene record
  2056
• Pubmed identfication
  • 9799793
  • 3838366
• RefSeq identity
  • NM_000799
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000152121

Gene info

• Identity
  HGNC:11820
• Gene
  TIMP1
• Long gene name
  TIMP metallopeptidase inhibitor 1
• Synonyms gene
  • TIMP
  • CLGI
• Synonyms gene name
  • tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
• Synonyms
  • EPO
• Locus
  Xp11.3
• Discovery year
  1986-01-01
• Entrez gene record
  7076
• RefSeq identity
  • NM_003254
• Classification
  • Tissue inhibitor of metallopeptidases
• VEGA ID
  OTTHUMG00000021447
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:3423
• Gene
  EPX
• Long gene name
  eosinophil peroxidase
• Synonyms
  • EPO
  • EPP
  • EPX-PEN
• GenBank acession
  • M26515
• Locus
  17q22
• Discovery year
  1998-11-24
• Entrez gene record
  8288
• Pubmed identfication
  • 2550461
  • 2541222
• RefSeq identity
  • NM_000502
• VEGA ID
  OTTHUMG00000178802

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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