Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit
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Catalog number
K758-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method - Fluorescence (Ex/Em = 380/500 nm) • Application: - Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity. - Removing tag from recombinant proteins having recognition motif.
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Features Benefits
• Simple & convenient procedure. • Can detect enteropeptidase activity as low as 1 mU. • Kit contains all necessary reagents for measuring enteropeptidase activity.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Biovision’s Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
Enteropeptidase/Enterokinase Activity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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