Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

  • Catalog number
    K758-100
  • Price
    Please ask
  • Size
    100 assays
  • Summary
    • Detection method - Fluorescence (Ex/Em = 380/500 nm) • Application: - Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity. - Removing tag from recombinant proteins having recognition motif.
  • Features Benefits
    • Simple & convenient procedure. • Can detect enteropeptidase activity as low as 1 mU. • Kit contains all necessary reagents for measuring enteropeptidase activity.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Biovision’s Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
  • Short name
    Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    Enteropeptidase/Enterokinase Activity fluorescence test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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