E2F2 Antibody

• Catalog number
  R30908
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  E2F2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the E2F2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This E2F2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q14209
• Purity
  Antigen affinity
• Description
  E2F transcription factor 2, also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.
• Immunogen
  An amino acid sequence from the C-terminus of human E2F transcription factor 2 (ISDLFDSYDLGDLLIN) was used as the immunogen for this E2F2 antibody (100% mouse homology).
• Storage
  After reconstitution, the E2F2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  E2F2  
• Gene symbol
  E2F2
• Short name
  Anti-E2F2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to E2F2
• Alternative technique
  antibodies
• Alternative to gene target
  E2F transcription factor 2, E2F-2, E2F2 and IDBG-93950 and ENSG00000007968 and 1870, transcription factor binding, nuclei, E2f2 and IDBG-197705 and ENSMUSG00000018983 and 242705, E2F2 and IDBG-645457 and ENSBTAG00000014400 and 617024

Gene info

• Identity
  HGNC:3114
• Gene
  E2F2
• Long gene name
  E2F transcription factor 2
• Synonyms
  • E2F-2
• GenBank acession
  • L22846
• Locus
  1p36.12
• Discovery year
  1994-08-31
• Entrez gene record
  1870
• Pubmed identfication
  • 8246995
  • 8246996
• RefSeq identity
  • NM_004091
• Classification
  • E2F transcription factors
• VEGA ID
  OTTHUMG00000003223

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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