E2F1 (Ser364) Antibody, ALEXA FLUOR 647
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Catalog number
bs-4076R-A647
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Price
Please ask
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Size
100ul
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Long name
E2F1 (Ser364) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
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Also known as
Anti-E2F1 Ser364 PAb ALEXA FLUOR 647
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 647
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
E2F1 Ser364
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Specificity
This is a highly specific antibody against E2F1 Ser364.
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Modification
Phosphorylation
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Modification Site
Ser364
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
350-370/437
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364
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Gene ID Number
1869
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Swiss Prot
Q01094
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
650nm/665nm
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Synonyms
RBP3; E2F-1; RBAP1; RBBP3; Transcription factor E2F1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-E2F1 Ser364 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 647
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French translation
anticorps
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Gene target
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Gene symbol
E2F1
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Short name
Anti-E2F1 Ser364
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 647
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Alternative name
E2F1 (Ser364) Polyclonal Antibody, ALEXA FLUOR 647
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Alternative technique
antibodies
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Alternative to gene target
E2F transcription factor 1, E2F-1 and RBAP1 and RBBP3 and RBP3, E2F1 and IDBG-67214 and ENSG00000101412 and 1869, sequence-specific DNA binding, nuclei, E2f1 and IDBG-210416 and ENSMUSG00000027490 and 13555, BT.78028 and IDBG-641855 and ENSBTAG00000003971 and 535369
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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