DYKDDDDK-Tag (3B9) mAb (Same as Sigma's Anti-FLAG)

• Catalog number
  GEN435047
• Price
  Please ask
• Size
  1 mililiter

• Also known as
  DYKDDDDK-Tag (3B9)
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Mouse IgG2b
• Clone
  N/A
• Host organism
  N/A
• Species reactivity
  All; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  N/A
• Concentration
  N/A
• Storage and shipping
  For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Stable for one year from the date of shipment.
• Tested applications
  Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag

• Gene target
  DYKDDDDK-Tag   3B9   mAb   Same   Sigma's  
• Short name
  DYKDDDDK-Tag (3B9) mAb (Same as Sigma's Anti- )
• Technique
  anti-, anti, antibody to, antibodies, Tags
• Label
  tag
• Alternative name
  Anti-DYKDDDDK-Tag (3B9) mAb (Same as Sigma's Anti-FLAG)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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