Dulbecco's Modified Eagle Medium w/ high-glucose, L-glutamine, and 25mM HEPES w/o sodium pyruvate and phenol red

• Catalog number
  DML12-500ML
• Price
  Please ask
• Size
  500 mL

• Availability
  Please contact us to check current availability
• Long description
  Dulbecco's Modified Eagle Medium w/ high-glucose, L-glutamine, and 25mM HEPES w/o sodium pyruvate and phenol red Medium
• Product format
  Liquid
• Product category
  Animal Cell Culture Media
• Product subcategory
  Dulbecco's Modified Eagle Medium
• Shipping method
  Ambient temperature
• Storage method
  Store at 2˚ to 8˚C.
• Other products
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Description
  There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Group
  media
• Conjugation
  red dye

• Gene target
  Dulbecco's   Modified   Eagle   glucose   L-glutamine   25mM   w/o   sodium   pyruvate   phenol   red  
• Short name
  DMEM (HG)
• Technique
  Medium, cell culture Media
• Label
  Red
• Alternative name
  Dulbecco's Modified Eagle Medium w/ high-glucose, L-glutamine, and 25mM HEPES w/o sodium pyruvate and phenol red
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  In Vitro Techniques
• Concept
  Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
• Tree numbers
  • E05.481
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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