DR3 (Death Receptor 3/Apo3) Polyclonal Antibody

• Catalog number
  3057-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  DR3 (Death Receptor 3/Apo3)
• Target Alternative Name
  Apo 3, LARD, TNFRSF12, TRAMP, TNFRSF25, WSL1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  Synthetic peptide corresponding to residues surrounding amino acids 400 of human DR3.
• Accession
  Q29905
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) antigen affinity-purified rabbit anti-DR3/Apo3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified rabbit IgG
• Application
  Western blot
• Application And Usages
  Western blot: 1-4 µg
• Description
  Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily through interaction with their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1, Apo3, TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1, overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD, TRAF2, and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis through TRADD, FADD, and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL), thymus and spleen.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• Additional description
  The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• French translation
  anticorps

• Gene target
  DR3   Death   Receptor   3/Apo3  
• Gene symbol
  TNFRSF25
• Short name
  DR3 (Death Receptor 3/Apo3) Polyclonal Antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  rabbit IgG
• Alternative name
  DR3 (Death Receptor 3/Apo3) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:11910
• Gene
  TNFRSF25
• Long gene name
  TNF receptor superfamily member 25
• Synonyms gene
  • TNFRSF12
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
  • tumor necrosis factor receptor superfamily, member 25
• Synonyms
  • DR3
  • TRAMP
  • WSL-1
  • LARD
  • WSL-LR
  • DDR3
  • TR3
  • APO-3
• GenBank acession
  • U72763
• Locus
  1p36.31
• Discovery year
  1998-12-04
• Entrez gene record
  8718
• Pubmed identfication
  • 9052839
  • 8934525
• RefSeq identity
  • NM_148965
• Classification
  • Tumor necrosis factor receptor superfamily
• VEGA ID
  OTTHUMG00000000831

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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