Donkey, DyLight® 550 conjugated

  • Catalog number
    AS12 1960
  • Price
    Please ask
  • Size
    1 mg
  • Available ordering format
    Lyophilized
  • Immunogen
    Purified rabbit IgG, whole molecule
  • Raised in
    Donkey
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified donkey IgG
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other dylight anti-rabbit secondary antibodies
  • Recommended dilutions for use
    1:20 - 1:2,000 for most applications
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
  • References
    to be added when available
  • Scientific context
    Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight®550 Conjugate is a secondary antibody conjugated to DyLight® 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
  • Notes
    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Latin name
    Equus africanus asinus
  • Gene target
  • Short name
    , DyLight® 550 conjugated
  • Technique
    antibody Conjugates
  • Host
    Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Label
    DyLight
  • Species
    Donkeys
  • Alternative name
    Donkey, DyLight® 550 coupled
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