Donkey F(ab)2 Anti-Rat IgG(H+L), Mouse SP ads-HRP
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Catalog number
MBS670692
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Price
Please ask
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Size
1 mL
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Products_type
Secondary Antibody
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Products_short_name
[Donkey F(ab)2 Anti-Rat IgG(H+L), Mouse SP ads]
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Products_gene_name
[IgG]
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Specificity
Reacts with the heavy and light chains of rat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other rat immunoglobulins. Minimal cross reaction to mouse serum proteins.
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Purity
Gel filtration chromatography of pepsin digested antibody
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Storage_stability
Store at 2-8 degree C
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Tested_application
ELISA (EIA)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Mus musculus, Rattus norvegicus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
F(ab)2 Anti- IgG(H+L), Mouse SP ads-
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Technique
anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgG, IgG
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Label
HRP
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Species
Mouse, Donkeys, Mouses, Rats
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Alternative name
Donkey binding fragment 2 Antibody toRat Immunoglobulin G(heavy + light chain), Mouse SP ads-horseradish peroxidase
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Alternative technique
murine, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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