Dnmt3b Antibody

• Catalog number
  F40611-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Dnmt3b
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:2000,IHC (Paraffin): 1:10-1:50
• Notes
  Titration of the Dnmt3b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Dnmt3b antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9UBC3
• Purity
  Purified
• Description
  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
• Immunogen
  A portion of amino acids 389-417 from the human protein was used as the immunogen for this Dnmt3b antibody.
• Storage
  Aliquot the Dnmt3b antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Dnmt3b  
• Gene symbol
  DNMT3B
• Short name
  Anti-Dnmt3b
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Dnmt3b
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2979
• Gene
  DNMT3B
• Long gene name
  DNA methyltransferase 3 beta
• Synonyms gene name
  • DNA (cytosine-5-)-methyltransferase 3 beta
• Locus
  20q11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1789
• Pubmed identfication
  • 9662389
  • 10433969
• RefSeq identity
  • NM_006892
• Classification
  • PWWP domain containing
  • 7BS C5-cytosine DNA/RNA methyltransferases
• VEGA ID
  OTTHUMG00000032226
• Locus Specific Databases
  • DNMT3Bbase: Mutation registry for ICF syndrome
  • LRG_56

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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