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Available ordering format
Liquid
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Immunogen
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG1 (clone N1664A)
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C for one year. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA),immuno-spin trapping (IT), western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP)
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Connected products
collection of antibodies to proteins involved in oxidative stressSecondary antibodies
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Recommended dilutions for use
1-10 µg/ml with standard ECL (WB), 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP)
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Molecular weight expected аpparent
90 kDa
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Verified reactivity
DMPO (species independent)
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Possible reactivity
DMPO (species independent)
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.
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References
Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
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Scientific context
DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
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Notes
Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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