DMPO, DMPO nitrone adduct

  • Catalog number
    AS11 1632
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Liquid
  • Immunogen
    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
  • Raised in
    Mouse
  • Clonality
    Monoclonal
  • Clone
    IgG1 (clone N1664A)
  • Purification
    Affinity purified
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at -20°C for one year. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    ELISA (ELISA),immuno-spin trapping (IT), western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP)
  • Connected products
    collection of antibodies to proteins involved in oxidative stressSecondary antibodies
  • Recommended dilutions for use
    1-10 µg/ml with standard ECL (WB), 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP)
  • Molecular weight expected аpparent
    90 kDa
  • Verified reactivity
    DMPO (species independent)
  • Possible reactivity
    DMPO (species independent)
  • No reactivity
    no confirmed exceptions from predicted reactivity are currently known
  • Supplementary information
    Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.
  • References
    Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
  • Scientific context
    DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
  • Notes
    Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Gene target
    DMPO   DMPO   nitrone   adduct  
  • Short name
    DMPO, DMPO nitrone adduct
  • Alternative name
    DMPO, DMPO nitrone adduct
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