DMEM - F12 w/ stable Glutamine w/ 15 mM Hepes - 500ml

• Catalog number
  LM-D1222/500
• Price
  Please ask
• Size
  25X500ml

• Description
  DMEM - F12 w/ stable Glutamine w/ 15 mM Hepes - 500ml is used in the maintenance and the culturing of mammalian cells. It offers excellent performance for certain epithelial, endothelial and granulose cell types.
• Similar product
• Shipping conditions
  -20°C or dry ice
• Storage
  +2/+8°C
• Shelflife
  24 months
• State
  Liquid
• Chemical description
  Water-based mixture of inorganic and organic compounds
• Appearance
  Physical state at 20 °C : Liquid ; Appearance: Fluid ; Colour : Red
• Density at 20 ºC
  1038 kg/m³
• Relative density at 20 ºC
  1.038
• PH
  7.4 ± 0.3
• Osmolality
  299 mOsm/kg ±10%
• Endotoxin
  < 1 EU/ml
• Cell growth test
  Medium tested for the ability to support Hela cell growth.
• Composition
  NA
• Sterility
  sterile filtered
• Personal precautions
  Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
• Uses
  Usage biotechnologique. Uniquement pour usage professionnel.
• Technical data
• Data sheet
• Ordering
  To order DMEM - F12 w/ stable Glutamine w/ 15 mM Hepes - 500ml, please use the Cat. Nr.LM-D1222/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for DMEM - F12 w/ stable Glutamine w/ 15 mM Hepes - 500ml
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  DMEM   F12   stable   Glutamine   500ml  
• Gene symbol
  F12
• Short name
  DMEM - F12 w/ stable Glutamine w/ 15 mM - 500ml
• Alternative name
  DMEM - F12 w/ stable Glutamine w/ 15 millimeter Hepes - 500ml

Gene info

• Identity
  HGNC:3530
• Gene
  F12
• Long gene name
  coagulation factor XII
• Synonyms gene name
  • coagulation factor XII (Hageman factor)
• GenBank acession
  • M31315
• Locus
  5q35.3
• Discovery year
  2001-06-22
• Entrez gene record
  2161
• RefSeq identity
  • NM_000505
• VEGA ID
  OTTHUMG00000163403
• Locus Specific Databases
  • LRG_145

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