Differential Buffered Charcoal Yeast Extract Agar Base

Description

Differential Buffered Charcoal Yeast Extract Agar Base is employed to isolate and differentiate Legionella species.
Other Product
Alternative Name

Differential BCYE Agar
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Yeast extract 10.000 Charcoal activated 1.500 L-Cysteine hydrochloride 0.400 Ferric pyrophosphate, soluble 0.250 ACES buffer 10.000 Alpha - Ketoglutarate 0.200 Bromocresol purple 0.010 Bromothymol blue 0.010 Agar 15.000 Final pH ( at 25°C) 6.9±0.2
Quantity required per litre

37,37 g
Appearance

Light grey to black homogeneous free flowing powder
Colour and Clarity of prepared medium

Grey-black coloured, opaque gel forms in Petri plates
Cultural response

Cultural characteristics observed with added 50 units/ml Polymyxin B and 1mg/ml Vancomycin, after an incubation at 35-37°C for 72-96 hours.
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

Pure extracts used in broths or as control for cell culture and Western Blot.

Gene target

Differential Buffered Charcoal Yeast Agar Base
Short name

Differential Buffered Charcoal Yeast Extract Agar Base
Technique

extract, extracts
Host

Yeast, Ascomycota
Species

Yeast, Yeasts
Alternative name

Differential Buffered Charcoal Yeast Extract media gelling agent Base
Alternative technique

extrats

Name

Electrophoresis, Agar Gel
Concept

Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Tree numbers
- E05.196.401.153
- E05.301.300.100
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data