DDX6 Antibody

• Catalog number
  R31069
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  DDX6
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the DDX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This DDX6 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P26196
• Purity
  Antigen affinity
• Description
  DEAD/H box 6, also known as RCK, HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. It belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp(DEAD) box motif(Seto et al., 1995). Tunnacliffe et al.(1993) assigned the gene more precisely using a panel of sequence tagged sites(STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that DDX6, EDC3 (YJDC), and HEDLS (RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, DDX6, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.
• Immunogen
  An amino acid sequence from the C-terminus of human DEAD/H box 6 (YDDRFNLKSIEEQL) was used as the immunogen for this DDX6 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the DDX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  DDX6  
• Gene symbol
  DDX6P1, DDX6
• Short name
  Anti-DDX6
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to DDX6
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:13948
• Gene
  DDX6P1
• Long gene name
  DEAD-box helicase 6 pseudogene 1
• Synonyms gene
  • DDX6P
• Synonyms gene name
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 pseudogene
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 pseudogene
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 pseudogene 1
  • DEAD (Asp-Glu-Ala-Asp) box helicase 6 pseudogene 1
• Synonyms
  • DDX6-Lp
  • bA150A6.3
• Locus
  6p22.1
• Discovery year
  2003-11-26
• Entrez gene record
  442192
• VEGA ID
  OTTHUMG00000031293

Gene info

• Identity
  HGNC:2747
• Gene
  DDX6
• Long gene name
  DEAD-box helicase 6
• Synonyms gene
  • HLR2
• Synonyms gene name
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD (Asp-Glu-Ala-Asp) box helicase 6
• Synonyms
  • RCK
  • Rck/p54
• GenBank acession
  • D17532
• Locus
  11q23.3
• Discovery year
  1992-09-14
• Entrez gene record
  1656
• Pubmed identfication
  • 1579499
  • 11839790
• RefSeq identity
  • NM_004397
• Classification
  • DEAD-box helicases
• VEGA ID
  OTTHUMG00000166411

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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