Daxx Antibody
-
Catalog numberR32346
-
PricePlease ask
-
Size0.1mg
-
-
CategoryAntibody
-
Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenDaxx
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB, IHC-P, ICC
-
Recommended dilutionsWestern blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
-
NotesOptimal dilution of the Daxx antibody should be determined by the researcher.
-
Intented useThis Daxx antibodyis to be used only for research purposes and not for diagnostics..
-
UniprotQ9UER7
-
PurityAntigen affinity
-
DescriptionDAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
-
ImmunogenAmino acids 56-345 of human Daxx were used as the immunogen for the Daxx antibody.
-
StorageAfter reconstitution, the Daxx antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
-
LocalizationNuclear and cytoplasmic
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolDAXX
-
Short nameAnti-Daxx
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameAntibodies to Daxx
-
Alternative techniqueantibodies
-
Alternative to gene targetdeath-domain associated protein, BING2 and DAP6 and EAP1, DAXX and IDBG-82726 and ENSG00000204209 and 102723452,1616, androgen receptor binding, nuclei, Daxx and IDBG-168138 and ENSMUSG00000002307 and 13163, DAXX and IDBG-629026 and ENSBTAG00000016830 and 504336
-
Gene info
-
Identity
-
Gene
-
Long gene namedeath domain associated protein
-
Synonyms gene name
- death-associated protein 6
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-03-25
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data