DAX-J2 Ratio 580/460
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Catalog number16310
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PricePlease ask
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Size1 mg
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DescriptionDAX-J2™ Ratio 580/460 is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells in a ratiometric mode under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to induce fluorescence ratio changes at wavelengths of 580 and 460 nm upon NO oxidation. The fluorescence intensities at 580 nm and 460 nm can be detected using the filter sets of Cy3®/TRITC and BD Horizon™ V450/Pacific Blue. These two filter sets are equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Ratio 580/460 has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-J2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Ratio 580/460 can be monitored in a ratiometric mode.
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ShippingRoom Temperature
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StorageFreezer
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Shelflife12 months
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Molecular Weight830.97
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AppearanceSolid
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SolubilitySoluble in: DMSO
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Excitation Wavelength420/540 nm
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Emission Wavelength460/580 nm
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Personal precautionsWear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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UsesLaboratory chemicals *For Research Use Only*
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OrderingTo order DAX-J2 Ratio 580/460, please use the Cat. Nr.16310 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for DAX-J2 Ratio 580/460
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Gene target
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Gene symbolIGKJ2, TRGJ2
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Short nameDAX-J2 Ratio 580/460
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Alternative nameDAX-J2 Ratio 580/460
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa joining 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameT cell receptor gamma joining 2
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Synonyms gene
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Synonyms gene name
- TCRGJ2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- T cell receptor gamma locus at 7p14
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VEGA ID