DAG (Diacylglycerol) Assay Kit

Type of the product

Set of reagents
Product description

Our DAG (Diacylglycerol) Assay Set of reagents measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the Set of reagents’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
Shipping conditions

The product DAG (Diacylglycerol) Assay Kit must be shipped on dry ice to keep the activity and stability of the reagents.
Storage conditions

Store the entire kit at minus eighty degrees Celsius. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Tips

This product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
Test

Cellular biology labs supplies other types of Assays as 1.

Gene target

DAG Diacylglycerol Kit
Gene symbol

DAG1
Short name

DAG (Diacylglycerol) Assay Kit
Technique

Assay, assays
Host

Assay
Alternative name

DAG (Diacylglycerol) test reagent
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832