CytoSelect™ 8-Channel Endothelial Microfluidic Biochips
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Catalog number
CBA-004
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Price
Please ask
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Size
2 chips
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We send this reagant on
Room temperature
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The temperatureerature for storage is
Room temperature
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Cell Adhesion, Endothelial Microfluidic Biochips
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Use of the reagent
The CytoSelect™ 8-Channel Endothelial Biochip is a self-contained unit containing 8 channels with an inlet/outlet port at both ends of each channel. The channels of the 8-Channel Endothelial Biochip are first coated with one or more ligands, e.g. adhesion molecules such as collagen, fibrinogen, by dispensing the ligand into one of the ports. After an incubation period, endothelial cells such as HUVEC are added. Then the cells to be assayed are added using a microfluidic syringe pump at a specified flow rate that mimics in vivo shear stresses. Cell adhesion may be visualized by brightfield or phase contrast microscopy.
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Cellular use
Provides a microfluidic environment that closely mimics in vivo shear stresses
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Protocol information
Useful for measuring cell adhesion and related processes while providing more physiologically relevant data than traditional static assays
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Reagent properties
Coat channels with an adhesion molecule followed by endothelial cells such as HUVEC
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Living modified organisme in this reagent or LMO
Not applicable
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,42
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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