Cytokeratin 18 antibody FITC
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Catalog number
61R-C161aFT
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Price
Please ask
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Size
250 µl
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Cell Biology
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Type of Immunogen
Cytokeratin 18 antibody (FITC) was raised in mouse using human cytokeratin 18 from HeLa cytoskeletal preparation ..
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Raised in
Mouse
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Specificity
Human cytokeratin 18
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Cross Reactivity
Bovine, dog, hamster, human, mouse, pig, rat, sheep,
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Isotypes
IgG1
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Clone
Ks 18.04
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Conjugate Tag
FITC
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Method of Purification
Protein A affinity chromatography
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Form Buffer
Provided as a liquid, with 0.09% NaN3
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
FC; IHC-F; IHC-P
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Usage Recommendations
IHC-F: 1:10, IHC-P: 1:10
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Cytokeratin 18 antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Mouse monoclonal Cytokeratin 18 antibody (FITC)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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