Cytochrome P450 2C19 (CYP2C19) Activity Assay Kit (Fluorometric)
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Catalog numberK848-100
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PricePlease ask
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Size100 assays
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DescriptionHighly Sensitive; Includes human CYP2C19 - NADPH Reductase as positive control.
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Summary• Detection method - Fluorescence (Ex/Em 406/468 nm) • Species reactivity - Eukaryotes • Applications - • Rapid assessment of native/recombinant CYP2C19 activity in lysates or microsomal fractions prepared from tissues, cells. • Screening of drugs and novel ligands for interaction with native/recombinant CYP2C19.
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Detection MethodFluorescence (Ex/Em 406/468 nm)
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Species ReactivityEukaryotes
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Applications• Rapid assessment of native/recombinant CYP2C19 activity in lysates or microsomal fractions prepared from tissues, cells. • Screening of drugs and novel ligands for interaction with native/recombinant CYP2C19.
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Sample Type• Human liver microsomes and liver S9 fractions • Lysates of tissues and cultured cells, primary hepatocytes • Heterologously expressed recombinant CYP2C19 preparations
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Features Benefits• Simple, convenient, highly sensitive • Fluorescent assay enables easy determination of CYP2C19 activity in a variety of biological samples • The substrate shows low background and a high signal-to-noise ratio • Kit includes CYP2C19 inhibitor (+)-N-3-benzylnirvanol and a stable, recombinant human CYP2C19 co-expressed with NADPH Reductase as a positive control
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundCytochrome P450 2C19 (CYP2C19, EC 1.14.14.1) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. CYPs are membrane-bound hemeproteins responsible for Phase I biotransformation reactions, in which lipophilic drugs and other xenobiotic compounds are converted to more hydrophilic products to facilitate excretion from the body. CYP2C19 is primarily expressed in liver and intestinal tissue and catalyzes oxidation of neutral or weakly basic lipophilic molecules with 2-3 hydrogen bond donor/acceptor sites. Isoforms of the CYP2C subfamily are responsible for metabolism of nearly 20% of all small molecule drugs commonly used by humans. Polymorphisms in the human CYP2C19 gene have been implicated in clinical drug/drug interactions involving widely-prescribed drugs, including proton pump inhibitors, antiplatelet agents and anticonvulsants. In addition, for drugs whose pharmacological activity requires metabolism from a pro-drug form, CYP2C19 inhibition or allelic deficiency can lead to decreased drug efficacy. BioVision’s CYP2C19 Activity Assay Kit enables rapid measurement of native or recombinant CYP2C19 activity in biological samples such as liver microsomes. The assay utilizes a non-fluorescent CYP2C19 substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 406/468 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence. A highly-selective CYP2C19 inhibitor is provided for determination of CYP2C19 activity in heterogeneous biological samples, where other CYP isozymes may contribute to substrate metabolism. The inhibitor displays greater than 100-fold selectivity for CYP2C19 over other CYPs, ensuring targeted inhibition. CYP2C19 specific activity is calculated by running parallel reactions in the presence and absence of the selective inhibitor and subtracting any residual activity detected with the inhibitor present. The kit contains a complete set of reagents sufficient for performing 50 sets of paired reactions (in the presence and absence of inhibitor).
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolCYP2C19, CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
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Short nameCytochrome P450 2C19 (CYP2C19) Activity Assay Kit (Fluorometric)
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TechniqueAssay, assays
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HostAssay
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Alternative nameCytochrome P450 2C19 (CYP2C19) Activity test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 2 subfamily C member 19
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19
- cytochrome P450, family 2, subfamily C, polypeptide 19
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cytochrome P450 family 2
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 3 subfamily A member 7
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Synonyms gene name
- cytochrome P450, subfamily IIIA, polypeptide 7
- cytochrome P450, family 3, subfamily A, polypeptide 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-14
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 24 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
- cytochrome P450, family 24, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-09
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Entrez gene record
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Classification
- Cytochrome P450 family 24
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 1 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
- cytochrome P450, family 1, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cytochrome P450 family 1
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 51 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
- cytochrome P450, family 51, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 51
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 2 subfamily D member 6
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6
- cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2
- cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-04-07
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 2
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VEGA ID
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Locus Specific Databases
