Cytochrome B5A, human recombinant

  • Catalog number
    7871-1000
  • Price
    Please ask
  • Size
    1 mg
  • Synonyms
    Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5
  • Alternative_names
    Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5
  • Description
    ≥95% Pure Human recombinant Cytochrome B5A which can function as an electron carrier for Cytochrome P450 enzymes
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Molecular Weight
    15 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Concentration
    1 mg/ml
  • Appearance
    Liquid
  • Physical form description
    Sterile filtered, 1 mg/ml solution containing 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT
  • Reconstitution Instructions
    In 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT
  • Background Information
    Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). BioVision’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.
  • Amino acid sequence
    MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    Cytochrome   B5A  
  • Short name
    Cytochrome B5A, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    Cytochrome B5A, H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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