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Target antigen
CYP2E1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CYP2E1(100-118aa RGDLPAFHAHRDRGIIFNN).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the CYP2E1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The CYP2E1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.
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Related articles
1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987. 2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987. 3. Hayashi, S.; Watanabe, J.; Kawajiri, K. Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.
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Gene Name
CYP2E1
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Protein Name
Cytochrome P450 2E1
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Gene Full Name
cytochrome P450, family 2, subfamily E, polypeptide 1
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Synonyms
4-nitrophenol 2-hydroxylase antibody|CP2E1_HUMAN antibody|CPE 1 antibody|CPE1 antibody|CYP2E antibody|CYP2E1 antibody|CYPIIE1 antibody|Cytochrome P450 2E1 antibody|Cytochrome P450 family 2 subfamily E polypeptide 1 antibody|Cytochrome P450 isozyme 3A antibody|Cytochrome P450 LM3A antibody|Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1 antibody|Cytochrome P450 subfamily IIE polypeptide 1 antibody|Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody|cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1 antibody|Cytochrome P450, subfamily IIE antibody|Cytochrome P450-ALC antibody|Cytochrome P450-J antibody|Cytochrome P450RLM6 antibody|EC 1.14.13.- antibody|EC 1.14.13.n7 antibody|Ethanol-inducible P450 antibody|Flavoprotein linked monooxygenase antibody|Flavoprotein-linked monooxygenase antibody|MGC133529 antibody|Microsomal monooxygenase antibody|OTTHUMP00000020813 antibody|OTTHUMP00000046571 antibody|P450 ALC antibody|p450 antibody|P450 J antibody|P450-J antibody|P450C2E antibody|P450J antibody|P450RLM6 antibody|Xenobiotic monooxygenase antibody
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Uniprot ID
P05181
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
