Cycloscope B-All

• Catalog number
  7043
• Price
  Please ask
• Size
  20 Tests

• Category
  Other Reagents
• Long description
  Cycloscope B-ALL, is a kit used for the flow cytometric analysis of DNA cell contents in B-lineage acute lymphoblastic leukemia (B-ALL). This kit is mainly focused for DNA studies of leukemic blast cells from bone marrow samples of these patients. _x000B__x000B_Introduction:_x000B_Acute lymphoblastic leukemia is a disorder characterized by a clonal expansion of lymphoid progenitor cells arrested at different differentation steps whose progressive accumulation causes bone marrow involvement with more than 30% blast cells at diagnosis. According to the European Group for the Immunological Clasification of Leukemias, assignement of an ALL to the B-lineage is based on the expression on leukemic cells of at least two B lineage associated antigens: CD19, cytoplasmic CD79a (mb-1) or cytoplasmic CD22. DNA cell content studies by flow cytometry provide relevant information for the prognostic evaluation and follow-up of patients with acute lymphoblastic leukemia. Detection of hyperdiploid leukemic blast cells is an independent prognostic factor strongly associated with favourable clinical and biologic features (1-3); in addition it may be of great utility for the detection of minimal residual disease contributing to relapse prediction in these patients (4, 5). At present the clinical utility of cell cycle studies in B-lineage ALL still remains to be established (6).
• Antibody come from
  n/a
• Other description
  No other information available
• Clone
  not specified
• Antigen antibody binding interaction
  Cycloscope B-All
• Antibody is raised in
  see techfile
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Flow Cytometry
• Antibody s suited for
  PROCEDURE This kit has been optimized in order to identify leukemic blast cells in patients diagnosed of B-lineage ALL with the purpose of studing the existence of DNA aneuploidy and the proliferative rate (proportion of cells in G0/G1, S and G2/M phases of cell cycle) of this cell population. _x000B__x000B_GOALS 1. To study the cell cycle distribution of leukemic blast cells in patients with B-progenitor cell acute lymphoblastic leukemia. 2. To detect the presence of DNA aneuploidy in B-lineage ALL patients. The criteria for the definition of DNA aneuploidy is defined by the presence of two or more different peaks of cells in the G0/G1 cell cycle phases. 3. To detect minimal residual disease in B-ALL patients with DNA aneuploid blast cells who are in morphological complete remision.
• Storage
  4ºC
• Relevant references
  1. Trueworthy R, Shuster J, Look AT, Crist WM, Borowitz M, Carroll A, Frankel L, Harris M, Wagner H, Haggard M, Mosijczuk A, Pullen J, Steuber P, Land V. Ploidy of lymphoblast is the stronger predictor of treatment outcome in B-progenitor cell acute lymphoblastic leukemia of childhood: a pediatric oncology group study. J Clin Oncol 10: 606-613; 1992. _x000B__x000B_2. Pui CH, Raimondi SC, Dodge R, Rivera GK, Fuchs LAH, Abromowitch M, Look AT, Furman WL, Crist WM, Williams D. Prognostic importance of structural chromosomal abnormalities in children with hyperdiploid (>50 chromosomes) acute lymphoblastic leukemia. Blood, 73: 1963-67; 1989. _x000B__x000B_3. Pui CH, Dodge RK, Look AT, George SL, Rivera GK, Abromowitch M, Ochs J, Evans WE, Crist WM, Simone JV. Risk of adverse events in children completing treatment for acute lymphoblastic leukemia: St. Jude total therapy studies VIII, IX and X. Clin. Oncol. 9: 1341; 1991. _x000B__x000B_4. Nowak R, OelschlŠgel U, Hofmann R, Zengler H, Huhn R. Detection of aneuploid cells in acute lymphoblastic leukemia with flow cytometry before and after therapy. Leuk. Res. 18: 897-901; 1989. _x000B__x000B_5. Ciudad J, San Miguel JF, L—pez-Berges MC, Valverde B, Vidriales B, L—pez AMA, Gonz‡lez M, Garc’aSanz R, Orfao A. Immunophenotypic detection of minimal residual disease in acute lymphoblastic leukemia. In Acute leukemias VI. Prognosis factors and treatment strategies. BŸchner et al (eds). Springer-Verlag Berlin Heidelberg 1997. _x000B__x000B_6. Look AT, Melvin SL, Williams DL, Brodeur GM, Dahl GV, Kalwinsky DK, Murphy SB, Mauer AM. Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia. Blood 60: 959-967; 1982. _x000B__x000B_7. Kaspers GJL, Smets LA, Pieters R, Van Zantwijk CH, Van Wering ER, Veerman AJP. Favorable Prognosis of Hyperdiploid common acute lymphoblastic leukemia may be explained by sensitivity to antimetabolites and other drugs: results of an in vitro study. Blood 85: 751-756; 1995. _x000B__x000B__x000B_8-.Nygaard U, Larsen J, Kristensen TD, Wesenberg F, Jonsson OG, Carlsen NT, Forestier E, Kirchhoff M, Larsen JK, Schmiegelow K, Christensen IJ. Flow cytometric DNA index, G-band karyotyping, and comparative genomic hybridization in detection of high hyperdiploidy in childhood acute lymphoblastic leukemia. J Pediatr Hematol Oncol: 28:134-40 (2006)._x000B__x000B_9-.Shman TV, Savitski VP, Fedasenka UU, Aleinikova OV. Apoptosis and proliferation differences between CD34+ and CD34- leukemic subpopulations in childhood acute leukemia. Hematology. 2:1 (2007)._x000B__x000B_10-.Heerema NA, Raimondi SC, Anderson JR, Biegel J, Camitta BM, Cooley LD, Gaynon PS, Hirsch B, Magenis RE, McGavran L, Patil S, Pettenati MJ, Pullen J, Rao K, Roulston D, Schneider NR, Shuster JJ, Sanger W, Sutcliffe MJ, van Tuinen P, Watson MS, Carroll AJ. Specific extra chromosomes occur in a modal number dependent pattern in pediatric acute lymphoblastic leukemia.Genes Chromosomes Cancer.46:684-93 (2007).
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link

• Gene target
  Cycloscope   B-All  
• Short name
  Cycloscope B-All
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Cycloscope B-All

Similar products