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ELISA s application
For quantitative measurement of human HO-1 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
Human HO-1
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ELISA standard curve
Concentration(pg/ml) + 0 + 93.7 + 187.5 + 375 + 750 + 1500 + 3000 + 6000 O.D. + 0.020 + 0.063 + 0.111 + 0.201 + 0.383 + 0.845 + 1.465 + 2.276
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ELISA kit detection limits
93.7pg/ml to 6000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human HO-1
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);527;2085;3561;686;2664;3724 ELISA standard deviations 19;91.74;185.2;31.6;151.8;242.1 CV(%);3.6;4.4;5.2;4.6;5.7;6.5
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ELISA kit description
GENTAUR's human HO-1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for HO-1 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, M1-M288) and your samples are measured with a biotin conjugated polyclonal from goat specific for HO-1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human HO-1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human HO-1 antibodies;1 ST0000-10;Lyophilized recombinant human HO-1 standard;10ng/tube×2 AR1107;Biotinylated anti- human HO-1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human HO-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human HO-1 standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
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Antigen
E.coli, M1-M288
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Heme oxygenase 1
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Gene name
heme oxygenase 1
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Alternative
32 kD;bK286B10;D8Wsu38e;heat shock protein 32 kD;heat shock protein 32kD;Heat shock protein;Heme oxygenase (decycling) 1;Heme oxygenase 1;Hemox;HMOX 1;Hmox;HMOX1;HMOX1_HUMAN;HO 1;HO;HO-1;HO1;Hsp32
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Gen protein identity
P09601
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Entrez nr
3162
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
HMOX1
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Short name
HO-1/HMOX1 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens HO-1/heme oxygenase (decycling) 1 PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
heme oxygenase (decycling) 1, bK286B10 and HMOX1D and HO-1 and HSP32, HMOX1 and IDBG-5374 and ENSG00000100292 and 3162, metal ion binding, nuclei, Hmox1 and IDBG-168890 and ENSMUSG00000005413 and 15368, HMOX1 and IDBG-637915 and ENSBTAG00000015582 and 513221
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