CTGFL/WISP-2, Human Recombinant
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Catalog number7129-50
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PricePlease ask
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Size50 ug
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SynonymsConnective Tissue Growth Factor-Like protein, CCN5, CT-58
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Alternative_namesConnective Tissue Growth Factor-Like protein, CCN5, CT-58
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DescriptionStimulates mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types
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RecombinantYes
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SourceE. coli.
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Activity Specifications test methodDetermined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
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Biological activityDetermined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
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Molecular Weight24.3 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized with no additives.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationCTGFL/WISP-2 is a 28.6 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors. Recombinant human CTGFL/WISP-2 is a 24.3 kDa protein of 228 amino acid residues.
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Amino acid sequenceMQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCCN5, CCN6, CCN4, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2
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Short nameCTGFL/WISP-2, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameCTGFL/WISP-2, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namecellular communication network factor 5
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Synonyms gene
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Synonyms gene name
- WNT1 inducible signaling pathway protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cellular communication network factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecellular communication network factor 6
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Synonyms gene
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Synonyms gene name
- WNT1 inducible signaling pathway protein 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cellular communication network factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecellular communication network factor 4
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Synonyms gene
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Synonyms gene name
- WISP1 overlapping transcript 1
- WISP1 3'UTR-associated RNA 1
- WISP1 overlapping transcript 1 (non-protein coding)
- WNT1 inducible signaling pathway protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cellular communication network factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR515 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR329 family
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 2
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data