CTCFL Antibody / BORIS

• Catalog number
  F53829-0.05ML
• Price
  Please ask
• Size
  0.05 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CTCFL / BORIS
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 1:1000-2000
• Intented use
  This CTCFL antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  140690
• Uniprot
  Q8NI51
• Purity
  Antigen affinity
• Description
  Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
• Immunogen
  A portion of amino acids 617-650 from the human protein was used as the immunogen for the CTCFL antibody.
• Storage
  Aliquot the CTCFL antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CTCFL   BORIS  
• Gene symbol
  CTCFL
• Short name
  Anti- CTCFL / BORIS
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to CTCFL / BORIS
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16234
• Gene
  CTCFL
• Long gene name
  CCCTC-binding factor like
• Synonyms gene name
  • CCCTC-binding factor (zinc finger protein)-like
• Synonyms
  • dJ579F20.2
  • BORIS
  • CT27
• Synonyms name
  • cancer/testis antigen 27
  • transcriptional repressor CTCFL
• Locus
  20q13.31
• Discovery year
  2001-07-17
• Entrez gene record
  140690
• RefSeq identity
  • NM_080618
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000032829

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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