CSP41b, ribosome associated endonuclease (CRB)

  • Catalog number
    AS08 298
  • Price
    Please ask
  • Size
    200 µg
  • Available ordering format
    Lyophilized in PBS pH 7.4
  • Immunogen
    KLH-conjugated synthetic peptide derived from CSP41b protein sequence of Arabidopsis thaliana Q9SA52, At1g09340
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified serum
  • How to reconstitute
    For reconstitution add 200 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of antibodies to various chloroplastic proteins
  • Recommended dilutions for use
    1: 1000 with standard ECL (WB)
  • Molecular weight expected аpparent
    42 | 39 kDa
  • Verified reactivity
    Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
  • Possible reactivity
    dicots including: Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchenis, Populus sp.,
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    to be added when available
  • References
    Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
  • Scientific context
    CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.
  • Notes
    None
  • Protein number
    Q9SA52
  • TAIR number
    AT1G09340
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