GM-CSF Recombinant Protein

Storage

For short term storage GM-CSF Recombinant Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
Tips

Due to handling and transportation, small amounts of the GM-CSF Recombinant Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
Gene

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

GM-CSF Protein
Gene symbol

CSF2, PPP1R3A, CSF1, CSF3, IL3
Short name

GM-CSF Recombinant Protein
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. aviva advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

GM-CSF Recombinant peptide
Alternative technique

rec

Identity

HGNC:9291
Gene

PPP1R3A
Long gene name

protein phosphatase 1 regulatory subunit 3A
Synonyms gene
- PPP1R3
Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 3A (glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle)
- protein phosphatase 1, regulatory (inhibitor) subunit 3A
- protein phosphatase 1, regulatory subunit 3A
Synonyms
- GM
Synonyms name
- glycogen-associated regulatory subunit of protein phosphatase-1
- protein phosphatase 1 regulatory subunit GM
GenBank acession
- AF024578
Locus

7q31.1
Discovery year

1993-01-22
Entrez gene record

5506
Pubmed identfication
- 7926294
RefSeq identity
- NM_002711
Classification
- Protein phosphatase 1 regulatory subunits
VEGA ID

OTTHUMG00000156944

Identity

HGNC:2438
Gene

CSF3
Long gene name

colony stimulating factor 3
Synonyms gene
- GCSF
- G-CSF
- C17orf33
Synonyms gene name
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
Synonyms
- MGC45931
Synonyms name
Locus

17q21.1
Discovery year

2001-06-22
Entrez gene record

1440
Pubmed identfication
- 3499671
- 3501046
RefSeq identity
- NM_172220
Classification
- Receptor ligands
VEGA ID

OTTHUMG00000133247

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data