G-CSF, Granulocyte colony-stimulating factor, Biotin conjugated

  • Catalog number
    IMS06-119-317
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
  • Immunogen
    purified full length recombinant human protein P09919
  • Raised in
    Hen
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgY
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
  • Verified applications
    Western blot (WB)
  • Connected products
    IMS01-119-316 | Granulocyte colony-stimulating factorIMS01-120-318 | Granulocyte-macrophage colony-stimulating factor
  • Recommended dilutions for use
    1: 5 000 with standard ECL (WB)
  • Molecular weight expected аpparent
    22 kDa
  • Verified reactivity
    human
  • Possible reactivity
    bovine, cat
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    to be added when available
  • References
    to be added when available
  • Scientific context
    Granulocyte colony-stimulating factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
  • Notes
    the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin.Total IgY concentration for this antibody is 15 mg/ml
  • Protein number
    P09919
  • TAIR number
    Refer to NCBI
  • Hyperlink
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Description
    Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Gene
    Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim.
  • Gene target
    G-CSF   Granulocyte   colony-stimulating   factor   conjugated  
  • Gene symbol
    CSF3, IGHV3-71, HLA-G, NCAPG, FOLR3, CSF2, SNRPG, GNAO1, CSF1, SFTA2
  • Short name
    G-CSF, Granulocyte colony-stimulating factor, conjugated
  • Technique
    antibody Conjugates
  • Label
    Biotin
  • Alternative name
    G-CSF, Granulocyte colony-stimulating factor, biotinilated coupled
  • Tissue
    granulocyte
Gene info
Gene info
  • Identity
    HGNC:5621
  • Gene
    IGHV3-71
  • Long gene name
    immunoglobulin heavy variable 3-71 (pseudogene)
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable 3-71
    • immunoglobulin heavy variable 3-G pseudogene (provisional)
    • immunoglobulin heavy variable 3-G (provisional)
    • immunoglobulin heavy variable 3-71 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
    14q32.33
  • Discovery year
    2000-04-04
  • Entrez gene record
    28411
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
    OTTHUMG00000151881
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
    HGNC:4389
  • Gene
    GNAO1
  • Long gene name
    G protein subunit alpha o1
  • Synonyms gene name
    • guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
  • Synonyms
  • Locus
    16q13
  • Discovery year
    1988-04-24
  • Entrez gene record
    2775
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • G protein subunits alpha, group i
    • MicroRNA protein coding host genes
  • VEGA ID
    OTTHUMG00000133241
Gene info
Gene info
MeSH Data
  • Name
    Colony-Forming Units Assay
  • Concept
    Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
  • Tree numbers
    • E01.370.225.500.383
    • E05.200.500.383
    • E05.242.383
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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G CSF Granulocyte colony stimulating factor conjugated
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