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Available ordering format
Lyophilized
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Immunogen
recombinant CrPDAT1 without transmembrane domains, overexpressed in E.coli,
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 350 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
antibody collection to other Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 250 with standard ECL (WB) and load per well of up to 30 ug
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Molecular weight expected аpparent
140 kDa
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlamydomonas reinhardtii
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
to be added when available
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References
Yoon et al (2012). Phospholipid:Diacylglycerol Acyltransferase Is a Multifunctional Enzyme Involved in Membrane Lipid Turnover and Degradation While Synthesizing Triacylglycerol in the Unicellular Green Microalga Chlamydomonas reinhardtii. Plant Cell, Oct 2012.
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Scientific context
PDAT1 is an enzyme phospholipid: diacylglycerol acyltransferase, which catalyzes TAG (triglycerols) synthesis. It has a strong lipase activity with a broad substrate specificity.
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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