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Target antigen
CrkL
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL(286-303aa FPFTHVKIFDPQNPDENE), different from the related mouse and rat sequences by one amino acid.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the CrkL Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The CrkL Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
CRKL(v-crk sarcoma virus CT10 oncogene homolog(avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3(src homology) domains. By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al.(1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene. Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
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Related articles
1. Guris, D. L., Duester, G., Papaioannou, V. E., Imamoto, A. Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev. Cell 10: 81-92, 2006. 2. Moon, A. M., Guris, D. L., Seo, J., Li, L., Hammond, J., Talbot, A., Imamoto, A. Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev. Cell 10: 71-80, 2006. 3. Senechal, K., Halpern, J., Sawyers, C. L. The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J. Biol. Chem. 38: 23255-23261, 1996.
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Gene Name
CRKL
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Protein Name
Crk-like protein
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Gene Full Name
v-crk sarcoma virus CT10 oncogene homolog(avian)-like
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Synonyms
Crk L antibody|Crk like protein antibody|Crk-like protein antibody|Crkl antibody|CRKL_HUMAN antibody|Crkol antibody|HGNC:2363 antibody|Oncogene CrkL antibody|V crk avian sarcoma virus CT10 oncogene homolog like antibody|v crk sarcoma virus CT10 oncogene homolog(avian) like antibody|V crk sarcoma virus CT10 oncogene homolog avian like antibody
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Uniprot ID
P46109
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
