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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from known CPN60 sequences, including Arabidopsis thaliana UniProt P21238 . TAIR AT2G28000.The peptide is conserved in chloroplastic CPN60A1 but NOT the close relative CPN60A2.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
collection of antibodies to RUBISCOcollection of antibodies to proteins involved in photosynthesis Plant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
57.1 kDa (mature protein)
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Verified reactivity
Arabidopsis thaliana, Arabidopsis thaliana cell culture, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativm, Zea mays
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Possible reactivity
Aegilops squarrosa, Avicena marina, Brassica napus, Canavalia lineata, Narcissus pseudonarcissus, Oryza sativa, Ricinus communis, Trifolium pratense, Triticum aestivum
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No reactivity
cyanobacteria, algae
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Supplementary information
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References
to be added when available, antibody released in September 2013.
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Scientific context
CPN60 alpha 1 (chaperonin 60 subunit alpha 1, chloroplastic) binds small and large subunits of Rubisco. Assists in protein folding and required for proper chloroplast development. Synonymes: protein SCHLEPPERLESS, RuBisCO large subunit-binding protein subunit alpha 1.
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Notes
The antibody will work on loads from 5 µg/well.
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Protein number
Refer to NCBI
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TAIR number
AT2G28000
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Description
The CPN60A1, Chaperonin 60 subunit alpha 1, chloroplastic is a α- or alpha protein sometimes glycoprotein present in blood.