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Synonyms
Core Histones (Human)
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Alternative_names
HeLa Core Histones
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Description
Mixture of human core histones involved in gene transcription
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Recombinant
No
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Source
Highly purified mixture of human core histones (H2A, H2B, H3, and H4) isolated via hydroxyapatite chromatography from HeLa S3 (human cervical adenocarcinoma) nuclear pellet.
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
6 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
50 mM sodium phosphate, pH 6.9, containing 10 mM sodium chloride and 1 mM β-mercaptoethanol.
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Background Information
A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core.2 Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
