Met (c Met) Antibody, FITC Conjugated
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Catalog number
bs-0668R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
Met (c Met) Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Met (c-Met)
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Specificity
This is a highly specific antibody against Met (c-Met).
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
25-75/1390
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Subcellular location
Extracellular
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Source
KLH conjugated synthetic peptide derived from human Met (c-Met)
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Gene ID number
4233
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Swiss Prot
P08581
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Tested applications
FCM, IF(IHC-P)
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Recommended dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
HGFR; AUTS9; RCCP2; c-Met; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Anti-Met (c-Met)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-Met (c-Met) PAb FITC
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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