Human Lactate Dehydrogenase A Inhibitor Screening Kit (Colorimetric)

• Catalog number
  K492-100
• Price
  Please ask
• Size
  100 assays

• Description
  Industry's first kit to screen/study/characterize potential inhibitors of LDH
• Summary
  • Detection method - Absorbance (OD = 450 nm). • Applications-Screening/characterizing/studying potential inhibitors of Lactate Dehydrogenase A
• Detection Method
  Absorbance (OD = 450 nm).
• Species Reactivity
  N/A
• Applications
  Screening/characterizing/studying potential inhibitors of Lactate Dehydrogenase A
• Sample Type
  Small molecules
• Features Benefits
  • Simple and sensitive assay. Fast protocol - can be performed in 30 min. HTP adaptable.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The human oxidoreductase Lactate Dehydrogenase A (LDHA) (EC:1.1.1.27) is a key enzyme in lactic acid metabolism. It interconverts pyruvate and NADH to lactate and NAD+ respectively. In mammals, LDHA exists as a tetramer and is primarily found in skeletal muscle tissues. LDHA is a common marker to assay for tissue damage and is also known to promote tumor proliferation in breast, lung, and pancreatic cancer patients. In BioVision’s LDHA Inhibitor Screening Kit, LDHA catalyzes the conversion of lactate to an intermediate that interacts with a probe to develop a color that can be measured at OD 450 nm. In the presence of a LDHA specific inhibitor, the reaction is impeded. A LDHA Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is high-throughput adaptable and can be completed in less than 1 hr
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Lactate   Dehydrogenase   Inhibitor   Screening   Kit  
• Short name
  Lactate Dehydrogenase A Inhibitor Screening Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Species
  Human, Humans
• Alternative name
  H. sapiens Lactate Dehydrogenase A suppressor detection reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products