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Target antigen
cMyc
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Clonality
Monoclonal antibody
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Clone
9e10
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human
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Analyses
WB,IHC-P,ICC
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Immunogen
Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the cMyc Antibody ( 9E10)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The cMyc Antibody ( 9E10) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
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Related articles
1. Liao D, Thakur A, Wu J, Biliran H, Sarkar FH. Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy.Crit Rev Oncog. 2007;13(2):93-158. 2. Britton S, Salles B, Calsou P. c-MYC protein is degraded in response to UV irradiation.Cell Cycle. 2007 Oct 2;7(1) 3. Chow JM, Liu CR, Lin CP, Lee CN, Cheng YC, Lin S, Liu HE. Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.Exp Hematol. 2008 Feb;36(2):140-8. 4. Huang CY, Bredemeyer AL, Walker LM, Bassing CH, Sleckman BP. Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse.Eur J Immunol. 2008 Jan 14.
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Gene Name
MYC
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Protein Name
Myc proto-oncogene protein
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Gene Full Name
v-myc myelocytomatosis viral oncogene homolog(avian)
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Synonyms
C-Myc|bHLHe39|Proto-oncogene c-Myc|Transcription factor p64|Class E basic helix-loop-helix protein 39
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Uniprot ID
Human(P01106), Mouse(P01108), Rat(P09416)
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Entrez GeneID
Human(4609), Mouse(17869), Rat(24577)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
The c-MYC or myelocytomatosis gene is a transcription factor that is found in cancer and many anti-c-MYC antibodies are used for its detection.
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French translation
anticorps
