Cycloscope B-NHL - Flow Cytometry Kit

  • Catalog number
    X1051
  • Price
    Please ask
  • Size
    20 Tests
  • Category
    Other Reagents
  • Long description
    CYCLOSCOPE B-NHL, is a kit used for the flow cytometric analysis of DNA cell contents in B- cell non-HodgkinÕs Lymphoma (B-NHL) and B-lineage chronic lymphocytic leukemias (B-CLL). This kit is mainly focused for DNA studies of B-cells from bone marrow or peripheral blood samples of these patients._x000B__x000B_In recent years, DNA flow cytometry studies have extended from basic research to clinical laboratories. Thus, flow cytometry analysis of the distribution of the cell nuclei DNA contents is being widely used to estimate the cell cycle distribution and the existence of DNA aneuploidy of either normal and tumour cell populations. Cycloscope kits are based on the combination of DNA cell measurements together with the analysis of tumoral cell antigen expression. This double staining method allows the identification of the neoplastic cells present in the sample in order to performe a DNA analysis separately from that of normal hemopoietic cells, as it is recommended by different consensus reports on DNA analysis by flow cytometry in neoplastic hematopathology._x000B__x000B_Previous studies have demonstrated that the percentage of tumor B-cells in S-phase have a clear role as an independent prognostic factor either in non-Hodgkin`s lymphoma or in chronic lymphocytic leukemia. On the other hand, it has been shown that DNA-ploidy correlates well with histopathologic grade in non-HodgkinÕs lymphoma patients.
  • Antibody come from
    n/a
  • Other description
    No other information available
  • Clone
    not specified
  • Antigen antibody binding interaction
    Cycloscope B-NHL - Flow Cytometry Kit
  • Antibody is raised in
    see techfile
  • Antibody s reacts with
    see techfile
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Flow Cytometry
  • Antibody s suited for
    This kit has been optimized in order to identify tumoral B-cells in patients diagnosed of B-NHL and B-CLL with the purpose of studying the proliferative rate (proportion of cells in G0/G1, S and G2/M phases of cell cycle) and the existence of DNA aneuploidy of this cell population._x000B__x000B_GOALS_x000B_1). To study the cell cycle distribution of B-cells in patients with B-cell NHL and B-CLL. _x000B_2). To detect the presence of DNA aneuploidy in B-NHL and B-CLL patients. The criteria for the definition of DNA aneuploidy is defined by the presence of two or more different peaks of cells_x000B_in the G0/G1 cell cycle phases. _x000B_3). To detect minimal residual disease in B-NHL and B-CLL patients with DNA aneuploid B-_x000B_cells who are in morphological complete remision. _x000B_4) To evaluate disease extent (staging) in B-NHL patients with DNA aneuploid B-cells._x000B_CLINICAL UTILITY_x000B_1.- CELL CYCLE Prognostic evaluation of B-NHL and B-CLL patients. It has been described that the S-phase (S) fraction of tumoral B-cells is an important prognostic indicator in non-Hodgkin lymphomas and B-lineage chronic lymphocytic leukemias._x000B_2.- DNA ANEUPLOIDY _x000B_Â¥ Monitoring of minimal residual disease (for patients with DNA aneuploidy at diagnosis)_x000B_ - Enumeration of tumoral B-cells in samples from B-NHL and B-CLL patients who are in morphologic complete remission, including samples which will be used for autologous transplantation._x000B_ - Detection of aneuploid B-cells in peripheral blood and/or apheresis samples which will be used for autologous transplantation._x000B_Â¥ Evaluation of disease extent. Detection of aneuploid B-cells in peripheral blood and bone marrow samples in order to assess disease extent._x000B_SENSITIVITY_x000B_The lowest level for detection of DNA aneuploid B-cells in samples morphologic complete remission (minimal residual disease) is 1 to 5 DNA aneuploid cells among 10.000 normal cells.
  • Storage
    4ºC
  • Relevant references
    1-.Terstappen LWMM, Johnsen W, Segers-Nolten IMJ, Loken MR. Identification and Characterization of Normal Human Plasma Cells in Normal Human Bone Marow by High Resolution Flow Cytometry. Blood, 76:1739-1747 (1990)._x000B_2-.Orfao A, Ciudad J, Gonz‡lez M, San Miguel JF, Garc’a AR, L—pez-Berges MC, Ramos F, Del Ca–izo MC, Rios A, Sanz M, L—pez-Borrasca A. Prognostic value of S-phase white blood cell count in B-cell chronic lymphocytic leukemia. Leukemia, 6: 47-51 (1992)._x000B_3-. Duque RE, Andreeff M, Braylan RC, Diamond LW, Peiper SC. Consensus review of the clinical utility of ADN flow cytometry in neoplastic hematopathology. Cytometry, 14: 492-496 (1993)._x000B_4-. Stelzer GT, Marti G, Hurley A, McCoy P, Lovett EJ, Schwartz A. U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry, 30: 214-230 (1997).
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
  • Gene target
    Cycloscope   B-NHL   Kit  
  • Gene symbol
    SNRPB, ORM2, ERAL1, OR1B1, SPIB, EXPH5, RTEL1, HLA-B, RELB, RPS6KA4
  • Short name
    Cycloscope B-NHL - Kit
  • Technique
    Flow Cytometry, Flow
  • Isotype
    not specified
  • Label
    unlabeled
  • Alternative name
    Cycloscope B-NHL - Flow Cytometry reagent
  • Alternative technique
    kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    Era like 12S mitochondrial rRNA chaperone 1
  • Synonyms gene name
    • Era (E. coli G-protein homolog)-like 1
    • Era G-protein-like 1 (E. coli)
    • Era-like 12S mitochondrial rRNA chaperone 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-11-19
  • Entrez gene record
  • Pubmed identfication
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    olfactory receptor family 1 subfamily B member 1
  • Synonyms gene name
    • olfactory receptor, family 1, subfamily B, member 1 (gene/pseudogene)
    • olfactory receptor family 1 subfamily B member 1 (gene/pseudogene)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-12-09
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Olfactory receptors, family 1
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    Spi-B transcription factor
  • Synonyms gene name
    • Spi-B transcription factor (Spi-1/PU.1 related)
  • Synonyms
  • Locus
  • Discovery year
    1994-12-15
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • ETS transcription factor family
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    major histocompatibility complex, class I, B
  • Synonyms gene
  • Synonyms gene name
    • ankylosing spondylitis
  • GenBank acession
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • C1-set domain containing
    • Histocompatibility complex
    • MicroRNA protein coding host genes
  • VEGA ID
  • Locus Specific Databases
Gene info
  • Identity
  • Gene
  • Long gene name
    RELB proto-oncogene, NF-kB subunit
  • Synonyms gene name
    • v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1995-10-02
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • IPT domain containing
    • NF-kappa B complex subunits
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    ribosomal protein S6 kinase A4
  • Synonyms gene name
    • ribosomal protein S6 kinase, 90kD, polypeptide 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-03-10
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • AGC family kinases
    • MicroRNA protein coding host genes
    • MAPK activated protein kinases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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