-
Synonyms
CHK, Serine/threonine-protein kinase Chk1
-
Alternative_names
CHK, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
-
Description
Regulates cell cycle checkpoints and apoptosis
-
Recombinant
Yes
-
Source
Baculovirus (Sf9 insect cells)
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 uCi/assay).
-
Molecular Weight
82.0 kDa
-
Storage Temp
-80°C
-
Shipping
dry ice
-
Shelf Life
12 months
-
Concentration
0.1 mg/ml
-
Appearance
Liquid
-
Physical form description
Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol.).
-
Background Information
CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
