Detection limits 15.62-1000 pg/mL
Sensitivity limit 5,6 pg/mL
Samples to be analyzed Serum, plasma and other biological fluids.
Assay duration 3 hours
Verified applications ELISA
ELISA detection Colorimetric
Protein number Please refer to Uniprot
Gene number Please refer to GenBank
Gene name Fibroblast Growth Factor 4
Alternate gene name HBGF4; HST; HSTF1; KFGF; K-FGF; Heparin Secretory Transforming Protein 1; Kaposi Sarcoma Oncogene; Transforming Protein KS3; FGF-Related Oncogene
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 4 (FGF4). No significant cross-reactivity or interference between Fibroblast Growth Factor 4 (FGF4) and analogues was observed.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 4 (FGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 4 (FGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 4 (FGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 4 (FGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Short name Fibroblast Growth Factor 4 (FGF4) ELISA Kit, 96
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name Chicken Fibroblast Growth Factor 4 (fibroblast growth factor 4) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, fibroblast growth factor 4, HBGF-4 and HST and HST-1 and HSTF1 and K-FGF and KFGF, FGF4 and IDBG-62016 and ENSG00000075388 and 2249, heparin binding, nuclei, Fgf4 and IDBG-212951 and ENSMUSG00000050917 and 14175, BT.62944 and IDBG-645032 and ENSBTAG00000012563 and 618474