CGE1, Alfa-CGE1, chloroplastic GrpE homolog

  • Catalog number
    AS06 176
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    mature CGE1 protein of Chlamydomonas reinhardtii, UniProt: Q945T1, expressed with N-terminal hexahistidin tag in E. coli, purified with Ni-NTA
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 100 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB) immunoprecipitation (IP)
  • Connected products
    collection of antibodies to other heat shock proteinscollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1:10 000 with standard ECL (WB)
  • Molecular weight expected аpparent
    24 | 27-28 kDa (SDS PAGE)
  • Verified reactivity
    Chlamydomonas reinhardtii
  • Possible reactivity
    Chlamydomonas reinhardtii
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    to be added when available
  • References
    Schroda et al. (2001) The chloroplastic GrpE homolog of Chlamydomonas: two isoforms generated by differential splicing. Plant Cell 13, 2823-2839.
  • Scientific context
    CGE1 is a nuclear-encoded, chloroplast-targeted co-chaperone of chloroplast HSP70B. It forms dimers that interact with HSP70B in the ADP-state.
  • Notes
    None
  • Protein number
    Q945T1
  • TAIR number
    Refer to NCBI
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