GST Fluorometric Activity Assay Kit
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Catalog number
K260-100
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Price
Please ask
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Size
100 assays
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Description
Highly sensitive in vitro assay
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Summary
• Detection method- Fluorescence (Ex/Em 380/461 nm) • Species reactivity- Mammalian • Applications- GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detects < 0.5 mU.
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes only ~1-2 hours • Fast and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex/em = 380/461 nm) when reacts with glutathione. GST catalyzes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detects < 0.5 mU.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
MGST3
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Short name
GST Fluorometric Activity Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
GST fluorescence Activity test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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