Cell Meter Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

  • Catalog number
    22832
  • Price
    Please ask
  • Size
    100 Tests
  • Description
    Cell Meter Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry* is used for monitoring cell viability. It is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). This kit is optimized to monitor cell apoptosis using a flow cytometer at Cy5 channel (red fluorescence).
  • Shipping
    Blue Ice
  • Storage
    Freezer
  • Shelflife
    6 months
  • Chemical components
    Component A: Apopxin™ Deep Red ; Component B: Assay Buffer
  • Excitation Wavelength
    649 nm
  • Emission Wavelength
    660 nm
  • Personal precautions
    Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
  • Uses
    Laboratory chemicals *For Research Use Only*
  • Ordering
    To order Cell Meter Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*, please use the Cat. Nr.22832 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
  • Additional description
    This 1 is suited for programmed cell-death studies. For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
  • Test
    aat supplies other types of Assays as 1. Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
  • Conjugation
    red dye
  • Gene target
  • Short name
    Meter Phosphatidylserine Apoptosis Assay Kit *Deep Red Optimized for *
  • Technique
    Flow Cytometry, apoptosis, Assay, Flow, apoptosis pathway, assays
  • Host
    Assay
  • Label
    Red
  • Alternative name
    cellular Meter Phosphatidylserine programmed cellular destruction test reagent *Deep Red Fluorescence Optimized to measure Flow Cytometry*
  • Alternative technique
    celldeath, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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