Cell Meter NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*

• Catalog number
  22802
• Price
  Please ask
• Size
  100 Tests

• Description
  Cell Meter NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry* is used for monitoring cell viability. It is designed to monitor cell apoptosis through measuring the loss of the mitochondrial membrane potential.
• Similar product
• Shipping
  Room Temperature
• Storage
  Freezer
• Shelflife
  6 months
• Chemical components
  Component A: 200X MitoLite™ NIR in DMSO ; Component B: Assay Buffer
• Excitation Wavelength
  646 nm
• Emission Wavelength
  659 nm
• Personal precautions
  Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
• Uses
  Laboratory chemicals *For Research Use Only*
• Technical data
• Data sheet
• Ordering
  To order Cell Meter NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*, please use the Cat. Nr.22802 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*
• Test
  aat supplies other types of Assays as 1. Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
• Additional description
  For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

• Gene target
  Meter   NIR   Mitochondrion   Membrane   Potential   Kit   Optimized   for  
• Gene symbol
  NOC2L
• Short name
  Meter NIR Mitochondrion Potential Assay Kit *Optimized for *
• Technique
  Flow Cytometry, Assay, Flow, assays
• Host
  Assay
• Alternative name
  cellular Meter NIR Mitochondrion Membrane Potential test reagent *Optimized to measure Flow Cytometry*
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

Gene info

• Identity
  HGNC:24517
• Gene
  NOC2L
• Long gene name
  NOC2 like nucleolar associated transcriptional repressor
• Synonyms gene name
  • nucleolar complex associated 2 homolog (S. cerevisiae)
• Synonyms
  • DKFZP564C186
  • NET7
  • NET15
  • NIR
  • PPP1R112
• Synonyms name
  • novel INHAT repressor
  • protein phosphatase 1, regulatory subunit 12
• GenBank acession
  • AL050019
• Locus
  1p36.33
• Discovery year
  2005-08-01
• Entrez gene record
  26155
• Pubmed identfication
  • 20123734
  • 22363708
• RefSeq identity
  • NM_015658
• Classification
  • Armadillo like helical domain containing
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000040720

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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