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ELISA s application
For quantitative measurement of human E-Cadherin in cell culture supernatant, serum, plasma heparin , saliva and urine.
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Gene tested
Human E-Cadherin
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ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.021 + 0.079 + 0.142 + 0.266 + 0.483 + 0.838 + 1.510 + 2.124
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ELISA kit detection limits
156pg/ml to 10000pg/ml
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ELISA s sensitivity
< 15pg/ml
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Target of this ELISA
Natural and recombinant human E-Cadherin
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.05;3.18;6.37;1.12;3.61;7.19 ELISA standard deviations 0.059;0.188;0.401;0.068;0.235;0.518 CV(%);5.6;5.9;6.3;6.1;6.5;7.2
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ELISA kit description
GENTAUR's human E-Cadherin ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for E-Cadherin was used as capure antibodies on the ELISA test plate. The internal standards (NSO, D155-I707) and your samples are measured with a biotin conjugated polyclonal from goat specific for E-Cadherin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human E-Cadherin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human E-Cadherin antibodies;1 ST0000-10;Lyophilized recombinant human E-Cadherin standard;10ng/tubex2 AR1107;Biotinylated anti- human E-Cadherin antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). dd 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma(heparin), saliva or urine to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human E-Cadherin standard solution and each sample is measured in duplicate.
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Minutes of TMB incubation
20-25
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More gene info
E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
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Antigen
NSO, D155-I707
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Cadherin-1
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Gene name
cadherin 1, type 1, E-cadherin (epithelial)
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Alternative
Arc 1;_x000D_Cadherin 1;_x000D_cadherin 1 type 1 E-cadherin;_x000D__x000D__x000D_Cadherin1;_x000D_CAM 120/80;_x000D_CD 234;_x000D_CD324;_x000D_CD324 antigen;_x000D_CDH1;_x000D_CDHE;_x000D_ECAD;_x000D_Epithelial cadherin;_x000D_epithelial calcium dependant adhesion protein;_x000D_LCAM;_x000D_Liver cell adhesion molecule;_x000D_UVO;_x000D_Uvomorulin
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Gen protein identity
P12830
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Entrez nr
999
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
CDH1
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Short name
E-Cadherin PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens E-Cadherin PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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